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KMID : 1123920140280060621
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Korean Journal of Oriental Physiology and Pathology
2014 Volume.28 No. 6 p.621 ~ p.629
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Genotoxicity Study of GST Extract
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Lee Chul-Wha
Han Jong-Min
Lee Mi-Young
Jung In-Chul
Jin Mi-Rim
Kim Seung-Hyung
Park Yang-Chun
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Abstract
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This study aimed to evaluate the genotoxicity of GST (Gamisasangja-tang). For examining genotoxicity, we carried out bacterial reverse mutation assay, chromosome aberration assay, micronucleus induction test according to OECD guidelines. Bacterial reverse mutation assay: In GST treating group, regardless of existence S9 mix, revertant colonies counts appeared to be less than twice of negative control group and dose dependent increase. In positive control group, revertant colonies counts were shown to be more than twice of negative control croup. Chromosome aberration assay: All cell line showed repetition rate of abnormal chromosome aberration less than 5%, regardless of treating time, existence of S9 mix, and no significant change (p?0.05) compared with negative control group. Micronucleus induction test: Micronucleated polychromatic erythrocytes (MNPCE) repetition rate of Polychromatic erythrocytes (PCE) showed no significant changes compared with negative control group (p?0.05). PCE portion of total erythrocytes also showed no significant changes (p?0.05). Our results showed that GST didn’t induce any genotoxicity.
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KEYWORD
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Sasangja-tang (Shechuangzi-tang), genotoxicity, mutagenicity, chromosome aberration assay, micronucleus induction
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